The DISC1 protein is widely expressed in normal tissues.
It is involved in neurite outgrowth and cortical development through its interaction with a variety of proteins including lissencephaly-1 (LIS1), NudeE-like (NUDEL) and phosphodiesterase 4B.
In certain cases of schizophrenia the DISC1 gene has been found to be disrupted by a t(1;11) (q42.1;q14.3) translocation, and studies suggest that DISC1 could be a candidate susceptibility gene for this condition.
Applications:Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-2ug/ml detects bands of approximately 95-100kD in SK-N-SH cell lysate.
Immunocytochemistry: 5ug/mlImmunohistochemistry: Frozen sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Specificity：Recognizes disrupted in schizophrenia 1 (DISC1), 93kD protein located in the nucleus, cytoplasm and mitochondria. Species Crossreactivity: human and mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：An 18 amino acid peptide located near the center of human DISC1.
