84-3391-06　［取扱停止］Deoxyribonuclease II, CT (DNase II, DNaseII, Deoxyribonuclease 2, DNASE2) (Biotin) 100ul　D3201-04-Biotin

特徴

• DNAse II is an enzyme involved in both the condensation of chromatin and the fragmentation of DNA.
• DNase II is ubiquitously expressed in human tissues.
• Applications:Suitable for use in Immunohistochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry (paraffin): 5ug/ml.
• Requires antigen retrieval using heat treatment prior to staining paraffin sections.
• Sodium citrate, pH 6.0 is recommended.
• Western Blot: 0.5-1ug/ml.
• Decects a band of ~40kD in human tissue spleen cell lysate
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.