ATG4A (Autophagy-related protein 4 homolog A), otherwise known as APG4A/Cysteine protease ATG4A, a cytosolic cysteine protease and member of the peptidase C54 family, predominantly expressed in skeletal muscle and brain, which plays a central role in the cleavage of the C-Terminal region of the target proteins GABARAPL2, MAP1LC3 or GABARAP during autophagy.
AHP2220 has been shown to be suitable for use in immunoprecipitation using HEK-293T cell lysates.
Applications:Suitable for use in Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Immunoprecipitation: 5-10ugOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Specificity:Recognizes human ATG4A.
Isotype:IgG
Calc Applications Abbrev:IP
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide corresponding to aa100-114 from human ATG4A conjugated to KLH. The corresponding sequence differs by 2aa in mouse and rat.