UNG1 is a member of the uracil-DNA glycosylase and cyclin family.
Two isoforms of UNG exist, isoform 1 is expressed in the mitochondria of in skeletal muscle, heart and testicles while isoform 2 (UNG2) is more abundant in proliferating tissues and expressed in the nucleus.
Uracil residues can be misincorporated into DNA by DNA polymerase or by deamination of cytosine.
UNG1 is a major enzyme in base excision repair, excising the misincorporated uracil residues in a process crucial to increasing immunoglobulin diversity.
In association with activation-induced cytosine deaminase, UNG is also essential to the generation of strand breaks that initiate class switch recombination.
UNG is also involved in the innate immune response against retroviral infections, HIV-1 accessory protein Vpr induces the rapid degradation of UNG2.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Alkaline Phosphatase.
Specificity：Recognizes human uracil-DNA glycosylase 1 (UNG1). Species Crossreactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：14 amino acid peptide sequence near the amino terminus of human UNG1.
