Ubiquitin-conjugating enzyme E2N (UBC13) is a 16kD enzyme which is an important component of the TLR (Toll-like receptor) and IL-1R signalling pathway.
UBC13 is DNA-damage inducible protein which forms part of an enzyme complex, with UEV1A, that indirectly activates TAK1 via the ubiquitination of TRAF-6.
Ubiquitin modification of proteins is important for error-free DNA repair and for targeting of short-lived or abnormal proteins for degradation.
Applications:Suitable for use in Immunohistochemistry (paraffin) and Western Blot.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 2ug/mlWestern Blot: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Highly Purified
Purity：Purified by Ion Exchange chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Alkaline Phosphatase.
Specificity：Recognizes C-terminal region of human UBC13. Species Crossreactivity: Rat, Mouse.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 15aa located near the human UBC13 carboxy-terminus.
