Activation of apoptosis by CD253 occurs through binding to the death-signaling receptors DR4 (TRAILR1) and DR5 (TRAILR2), leading to formation of the death-inducing signaling complex (DISC) and the triggering of a proteolytic caspase cascade.
There are however decoy receptors known as DcR1 (TRAILR3) and DcR2 (TRAILR4) which competitively bind to CD253 and are unable to initiate apoptosis.
CD253 also plays a role in immune surveillance, acting as a mediator of monocyte, NK cell, dendritic cell and T cell anti-tumor and anti-viral cytotoxicity.
Applications:Suitable for use in ELISA, Immunohistochemistry (paraffin), and Western Blot.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 0.25ug/mlELISA: 0.5ug/mlWestern Blot: 0.1-0.2ug/mlRequires antigen retrieval using heat treatment for 20 minutes prior to staining of paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Alkaline Phosphatase.
