PS34 (vacuolar protein sorting 34), otherwise known as PI3-kinase type 3 (PIK3C3), is a ubiquitously expressed class III PI3K (phosphoinositide 3-kinase), and member of the PI3/PI4-kinase family, which specifically utilizes phosphatidylinositol (PtdIn) as a substrate, and functions as the catalytic subunit of the PI3K complex.
VPS34 plays a role in the transport of lysosomal enzyme precursors to lysosomes, and in endocytosis and vesicular trafficking, and has been strongly implicated as a regulator of autophagy, forming a multi-protein complex with the proautophagic tumor suppressors Beclin1/Atg6, Bif-1, and UVRAG, in the initiation of autophagosome formation.
Distinct complexes of VPS34 have been shown to regulate endocytic processes vital for late-stage autophagosome-lysosome fusion.
PS34 has been tested in immunofluorescence using HUVEC cells, in conjunction with Hoescht 33342 (blue) nuclear staining.
Applications:Suitable for use in and Western Blot.
Other applications not tested.
Recommended Dilution:1:100 Western Blot: 2-4ug/ml, detects a band of ~105kD in HEK-293T cell lysates
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Alkaline Phosphatase.
Specificity：Recognizes human PIK3C3. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa22-34 of human PIK3C3, KLH conjugated.
