PHAP I plays a role in a number of cellular processes, including proliferation, differentiation, apoptosis and tumor suppression.
It is expressed in a variety of tissues with highest expression in kidney and skeletal muscle.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: detects a band at ~32kD in Raji cell lysateImmunohistochemistry (Paraffin): 2ug/ml requires antigen retrieval using heat treatment prior to staining paraffin sections.
Sodium citrate buffer, pH 6.0 is recommended.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Alkaline Phosphatase.
Specificity：Recognizes human PHAP I. Has no cross-reaction to PHAP I2a and PHAP III. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 14 amino acids at the carboxy terminus of human PHAP I.
