PUMA, also known as bbc3, is a pro-apoptotic member of the BCL-2 family.
PUMA is localized to the mitochondria, binds BCL-2 and induces cytochrome C release and apoptosis in response to p53.
Applications:Suitable for use in Immunohistochemistry and Western Blot.
Other applictions not tested.
Recommended Dilutions:Western Blot: 2-4ug/ml.
Detects a band at ~23kD in K562 cell lysates
Immunohistochemistry (paraffin): 10ug/ml.
Requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Sodium citrate buffer pH 6.0 is recommended
Optimal working dilutions to be determined by researcher.
Positive Control:Human breast carcinoma
Recommended Secondary Reagents:Sheep anti Rabbit IgG (HRP)
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography from whole serum.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Alkaline Phosphatase.
Specificity：Recognizes the human p53-upregulated modulator of apoptosis (PUMA).
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide, ARARQEGSSPEPVEG corresponding to the N-terminus of human Bcl-2 binding component 3 (PUMA). Species sequence homology: mouse
