Human IL-17F (interleukin-17F), a disulphide-linked homodimer consisting of two 133aa chains, which belongs to the IL-17 gene family and shares the highest sequence homology with IL-17A.
IL-17F is expressed by activated CD4+ T-cells and monocytes, and acts as a stimulator of cytokine production, including granulocyte colony-stimulating factor (GM-CSF), IL-6 and IL-8.
IL-7F also stimulates the production of IL-2, MCP-1 (monocyte chemotactic protein-1) and TGFB (Transforming growth factor beta) by endothelial cells, and inhibits endothelial cell angiogenesis
Has been used in neutralisation assays where 50% inhibition (ND50) of the biological activity of 50ng/ml of human IL-17F was achieved using 0.9-1.2ug/ml of antibody.
Applications:Suitable for use in ELISA, Western Blot, Functional Assays.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-2ug/mlWestern Blot: 0.1-0.2ug/mlFunctional Assays: 0.9-1.2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Alkaline Phosphatase.
Specificity：Recognizes human Interleukin-17F.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human Interleukin-17F.
