84-3387-98　［取扱停止］Glycine Receptor alpha 1/2 (AP) 100ul　G8167-02-AP

特徴

• Glycine receptors are members of the ligand-gated ion channel superfamily, which mediate fast inhibitory neurotransmission.
• The receptors are pentameric membrane proteins which form chloride channels.
• Binding of glycine to its receptor produces an increase in chloride conductance and membrane hyperpolarisation.
• Four genes encoding glycine receptor alpha subunits have been identified, together with a single beta polypeptide.
• Each subunit consists of a large extracellular N-terminal region, four transmembrane domains, and a large cytoplasmic domain.
• Applications: Suitable for use in Western Blot and Immunohistochemistry (frozen).
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry: 1:1000Western Blot: 1:1000Optimal dilution determined by the researcher
• Recommended Secondary Reagents:Sheep anti rabbit IgG:HRP
• Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
• Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.
• Note: Applications are based on unconjugated antibody.