FLIP interacts with the adaptor protein FADD, and with Caspase-8 (FLICE) and Caspase-10 (FLICE2) to inhibit apoptosis induced by death receptors including CD95, DR3, TRAIL-R and TNFR1.
FLICE inhibitory proteins were initially identified in virus and human and were accordingly designated as v-FLIPs and c-FLIPs.
Four spice variants of c-FLIP have been identified and are classified as FLIP alpha, beta, delta and gamma.
F4550-50 recognizes human and murine delta and gamma variants but not the alpha or beta, variants.
FLIP is widely expressed in human tissues and a diverse range of cell lines.
Applications:Suitable for use in Immunocytochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Alkaline Phosphatase.
Specificity：Recognizes human FLIP gamma/delta, CT. Does not recognize the alpha or beta variants. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Peptide corresponding to aa191-209 from the C-terminus of human FLIP delta/FLIPs form.
