Specifically Recognizes an epitope within the C-terminal (CT) region of endothelin B receptor (ET-B), a widely distributed transmembrane protein and member of the G-protein coupled receptor 1 family, which acts as a non-specific receptor for endothelin 1, 2, and 3, and mediates its action by G protein association and activation of a phosphatidylinositol-calcium second messenger system.
Recognizes epitopes on the cytoplasmic side of the receptor and therefore the cell membrane must be permeable to the antibody.
Does not block the binding of endothelins
Applications:Suitable for use in Immunoprecipitation, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:10-20ug/mlImmunoprecipitation: 20-40ug/mlImmunohistochemistry(Paraffin): 10-20ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：sheep
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Alkaline Phosphatase.
Specificity：Recognizes rat Endothelin B Receptor. Species Crossreactivity: Mouse, human
Isotype：IgG
Calc Applications Abbrev：IHC IP
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to KANDHGYDNFRSSNN aa424-437 of rat Endothelin B Receptor, conjugated to KLH.
