Recommended Secondary Reagents: Sheep anti Rabbit IgG (Aff.
Pur.): HRP
Applications:Western Blotting: 1-2ug/mlOptimal working dilutions to be determined by researcher.
Western Blotting: Detects a band of approximately 35kD in HepG2 and 3T3 cell lysates.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified IgG was prepared from whole serum by affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. Labeled with Alkaline Phosphatase.
Specificity:Recognizes endonuclease G (endoG), a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis endoG i reported to act independently of caspases and may represent a caspase independent apoptotic pathway initiated from the mitochondria. Species Crossreactivity: Human. Expected crossreactivity from sequence similarity mouse, rat.
Isotype:IgG
Calc Applications Abbrev:WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:Synthetic peptide GGPRGPGELAKYGLP corresponding to amino acids 55 - 70 of endonuclease G.