Bim is a pro-apoptotic protein within the Bcl-2 family, which functions within the intrinsic apotosis pathway.
Bim is known to interact with pro-survival proteins including Bcl-2, Bcl-XL and Bcl-w.
Bim is expressed in a variety of cell types, where it is maintained in an inactive conformation by phosphorylation or through binding to the microtubule-associated dynein motor complex.
Upon certain apoptotic stimuli, Bim is released from microtubules and mediates caspase-dependent apoptosis.
Applications:Suitable for use in Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: detects a band of ~22kD in K562 and A549 whole cell lysates.
Immunohistochemistry: Paraffin sections.
Requires antigen retrival using heat treatment prior to staining of paraffin sections.
Sodium citrate pH 6 is recommended for antigen retrival
Optimal dilution determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Alkaline Phosphatase.
Specificity：Recognizes human BIM.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa22-40 of human BIM. Species Sequence Homology: mouse and rat.
