Sheep Anti 5-Methylcytosine (5-MeC) specifically recognizes the methylated form of cytosine (C), an epigenetic modification catalyzed by DNA methyltransferases resulting in the attachment of a methyl group to carbon 5.
This modification alters the structure of cytosine but not its base-pairing properties.
The timing and function of cytosine methylation varies amongst species with roles in such processing events as transcription and recombination.
For example, in mammals, methylation occuring during early development and emrbyogensis functions in X chromosome inactivation and gene imprinting, wherease in plants, DNA methylation occurs later and affects genomic stability through silencing of transposable elements.
The deamination of 5-MeC results in the formation of thymine (T) instead of uracil.
Since thymine is not recognized by DNA repair enzymes, cytosine to thymine conversions can cause transition mutations.
The most frequent mutations in human genetic diseases are those involving C to T transitions occurring at CG sites Another type of mutation involving 5-MeC involves the formation of pyrimidine dimers.
Upon exposure to sunlight, pyrimidine dimers form preferentially at dipyrimidine sequences containing 5-MeC, a mutation frequently seen in human skin cancers.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 25-100ug/mlImmunohistochemistry(Frozen/Paraffin): 10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：sheep
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Alkaline Phosphatase.
