MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4.
They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain.
VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells.
Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells.
KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling.
KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells.
Flt3 (also known as Flk-2) is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms.
It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Control:CSF-1 treated PAE cell lysate, transfected with CSF/KDR.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Specificity：Recognizes human VEGFR2 when phosphorylated on Tyrosine 1054. Species Crossreactivity: mouse, rat, primate, bovine, chicken, equine and zebrafish.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Bo Ch Eq Hu Mo Rt Ze
Immunogen：Synthetic peptide corresponding to VEGFR2 when phosphorylated on Tyrosine 1054.
