84-3386-01　［取扱停止］FOXO1, phosphorylated (Thr24) (FKHR, Forkhead Box Protein O1, Forkhead Box Protein O1A, Forkhead In Rhabdomyosarcoma, FOXO1A) (MaxLight 750) 100ul　029480-ML750

特徴

• MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
• Absorbance (759nm); Emission (780nm); Extinction Coefficient 240,000.
• Forkhead box O (FOXO) transcription factors play an important role in modulating metabolic functions and cell proliferation.
• Given the relatively high expression of FOXO1 in insulin-responsive tissues, this transcription factor is highly poised to regulate energy metabolism.
• When nutrient and insulin levels are low, FOXO1 promotes expression of gluconeogenic enzymes.
• Conversely, in the fed state, insulin levels rise and stimulate uptake of glucose primarily into skeletal muscle and other organs, including adipose tissue.
• Under certain pathophysiologic conditions, including insulin resistance, negative signaling to FOXO1 is compromised.
• Increased cell proliferation occurs when FOXO1 is inactivated when phosphorylated by Akt at 24aa.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: detects phospho-FOXO1 (Thr24) in HEK293 cell lysate untreated and BMP2-treated
• Optimal dilutions to be determined by the researcher.
• Positive Control:HEK293 cell lysate untreated and BMP2-treated
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: MaxLight™750 conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.