MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Dopamine- and cAMP-regulated neuronal phosphoprotein (DARP-32) is a cytoplasmic member of the protein phosphatase inhibitor 1 family.
DARP-32 is a multifunctional protein kinase and protein phosphatase regulator, depending on the site of its activation.
It possesses at least four sites that can be phosphorylated.
Phosphorylation by PKA at Thr34 stimulates DARP-32 to act as a protein-phosphatase-1 (PP-1) inhibitor.
However, phosphorylation at Thr75 will inhibit Thr34 phosphorylation.
This selective, bidirectional regulation allows DARPP-32 to have a wide range of influence on downstream physiological effectors such as: ion pumps, neurotransmitter receptors, voltage-gate ion channels, and transcriptional factors.
Studies suggest that it may also have a role in the Circadian System photic pathway.
Overexpression of DARPP-32 has been linked with antiapoptotic mechanisms of cancer cells and has been observed in breast, colon, esophagus, gastric, and prostate cancer tissues.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilutions: Western Blot: 1ug/ml detects DARPP-32 on 10ug of rat brain tissue lysate
Immunoprecipitation: 10ug immunoprecipitates DARPP-32 from rat brain RIPA lysate
Optimal dilutions to be determined by the researcher.
Positive Control:Rat brain tissue lysate
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Specificity：Recognizes mouse DARPP-32. Species crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Mo Rt
Immunogen：Linear peptide corresponding to mouse DARPP-32 near the N-terminus (KLH). Cellular Localization: cytoplasm. Species sequence homology: canine, Rhesus Macaque.
