MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Tbr1 gene is a member of a conserved family of genes that share a common DNA-binding domain, the T-box.
T-box genes encode transcription factors involved in the regulation of developmental processes.
Tbr1, a putative transcription factor known to be expressed in post-mitotic neurons, has been found highly expressed in glutamatergic early-born cortical neurons.
A similar protein has been disrupted in mice and shown to be critical for early cortical development, and causes loss of projection neurons in the olfactory bulbs and olfactory cortex.
The C-terminal region of the Tbr1 derived protein was found to be necessary and sufficient for association with the guanylate kinase domain of calcium/calmodulin-dependent serine protein kinase.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.025ug/ml detected TBR1 in 10ug of human fetal brain tissue lysate.
Human fetal brain tissue lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with T1848-23.
Proteins were visualized using a IgY (HRP) Rabbit x Ch* and a chemiluminescence detection system
Immunohistochemistry: 1:100 detected TRB1 in mouse frontal cortex tissue
Optimal dilutions to be determined by the researcher.
*Recommended Secondary Antibodies:I1906: IgY, Chicken Pab Rb x ChI1906-10: IgY, Chicken (HRP) Rb x ChI1906-12: IgY, Chicken (FITC) Rb x ChI1906-14: IgY, Chicken (AP) Rb x Ch
Positive Control:Human fetal brain tissue lysate
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：chicken
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Specificity：Recognizes mouse TBR1 at ~68kD. Species Crossreactivity: human and rat.
Isotype：IgY
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Linear peptide corresponding to mouse T-box Brain Protein 1 (TBR1), conjugated to KLH (NP_033348). Species Sequence Homology (100%): equine, porcine, opossum, bovine and canine.
