MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
LCN2 (also known as Neurophil Gelatinase-Associated Lipocalin or NGAL) belongs to a superfamily of lipocalins, a collection of small, diversely functional, extracellular proteins.
NGAL is secreted from neutrophil granules in three different forms; a monomer of 25 kDa, a disulfide-linked homodimer at 46 kDa, and a disulfide-linked heterodimer with MMP-9 at 135-kDa.
It is widely known to induce development of kidney epithelia, to bind and traffic iron, and to act as a molecular signal under inflammatory conditions, such as Crohn’s Disease.
Multiple carcinoma studies have also shown elevated expression levels of LCN2 in several different human tumors affecting such organs as: colon, lung, breast, ovaries and pancreas.
Additionally, there has been great interest in this protein as a possible marker for: onset of Acute Kidney Injury following cardiac surgery, the progression of Chronic Kidney Disease (CKD), and the survival chances of patients with Chronic Heart Failure (CHF).
Applications: Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Westrn Blot: 5ug/mlImmunohistochemistry: 1:200Optimal dilutions to be determined by the researcher.
Positive Control:Human kidney tissue lysate
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Specificity：Recognizes human LCN2.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Linear peptide corresponding to the mature chain of human LCN2 (KLH). Species sequence homology: mouse, bovine and rat.
