84-3385-71　［取扱停止］Patched 1 (PTCH, PTC, BCNS, FLJ42602, Holoprosencephaly 7, HPE7, NBCCS, Patched 1, Patched Homolog 1, Patched Protein Homolog 1, PTC1, PTCH1) (MaxLight 750) 100ul　P3113-82C4-ML750

特徴

• MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
• Absorbance (759nm); Emission (780nm); Extinction Coefficient 240,000.
• Patched 1 is a receptor for sonic hedgehog, a member of the hedgehog family of cell signaling proteins.
• Hedgehog signaling is regulated by Protein patched homolog 1 which keeps the pathway turned off in the absences of activation.
• This inhibits the function of associated downstream network components such as Smoothened (Smo).
• Applications:Suitable for use in Western Blot and Immunohistochemistry
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.1ug/ml detected Patched 1 on 10ug of human skeletal muscle, fetal tissue lysate
• Immunohistochemistry (Paraffin): 1:300 detected Patched 1 in kidney tissue.
• Heat induced epitope retrieval with citrate buffer, pH 6.0 is recommended
• Optimal dilutions to be determined by the researcher.
• Recommended Secondary Antibodies:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRbI1904-46Q: IgG, H&L (HRP) Pab Gt xRb
• Positive Control:Human skeletal muscle, fetal tissue lysate.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: MaxLight™750 conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.