MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
ASPM gene is the human ortholog of the Drosophila melanogaster 'abnormal spindle' gene (asp), which is essential for normal mitotic spindle function in embryonic neuroblasts (Bond et al., 2002 [PubMed 12355089]).
The mouse gene ASPM is expressed specifically in the primary sites of prenatal cerebral cortical neurogenesis.
Molecular Weight:218, 95, 75kD Observed UniProtKB/Swiss-Prot entry Q8IZT6 describes 2 isoforms produced by alternative splicing: Isoform 1 at 409.800kD and Isoform 2 at 217.794kD.
Multiple other isoforms in ref: Human Molecular Genetics, 2005, Vol.
14, No.
15 2155-2165.
Applications:Suitable for use in FLISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:FLISA: Tested in direct FLISA against the ASPM immunogen
Immunohistochemistry (Paraffin): 1:300.
Positive and background staining patterns on formalin fixed, paraffin embedded human ductal carcinoma.
Tissue pretreated with citrate pH 6.0 using antigen retrieval techniques.
Detected using HRP/DAB.
Immunoreactivity is seen in nucleus and cytoplasm
Optimal dilutions to be determined by the researcher.
Positive Control:Human ductal carcinoma tissue
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Specificity：Recognizes human ASPM. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：FLISA IHC
Calc Crossreactivity：Hu Rt
Immunogen：Linear peptide corresponding to human ASPM conjugated to KLH. (NP_060606). Epitope: Internal. Species Sequence Homology: chimpanzee and primate
