MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Phosphoinositol-3-kinases (PI3 Kinase, PI3K) are a family of lipid kinases that mediate many intracellular signaling responses in both physiological and pathophysiological states.
PI3 Kinase is a heteroduplex with three classes (I, II, & III) of catalytic domain.
The class I PI3 Kinases are made up of a p85 regulatory protein and a p110 catalytic domain.
There are 4 isoforms of the class I p110 catalytic domains.
Class I(A) include alpha, beta, gamma and class I(B) delta.
They are activated downstream via receptor tyrosine kinases or G-protein coupled receptors.
Once activated, PI3K generates phosphatidylinositol 3,4,5-trisphosphate (PIP3) from PIP2.
It was later found that PTEN, a lipid phosphatase, acts opposite to PI3K by dephosphorylating PIP3, converting it to PIP2, thus de-activating the effects of PI3K.
Increased levels of PIP3 in the cell leads to the activation of many key signaling regulators including Akt and PDK as well as calcium release.
One of the primary roles of PI3K is its regulation of cell growth and cell metabolism.
Both of these play a critical role in cancer as some believe that a tumor’s shift to glycolysis is due to the activation of PI3K.
PI3 kinases have been linked with numerous disease states, including cancer, diabetes, allergic response, hypertension, cardiac contractility, atherosclerosis, sepsis and autoimmune/inflammatory disorders.
Applications:Suitable for use in FLISA, Western Blot, Immunoprecipitation, and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Specificity：Recognizes human PI3 Kinase p110 alpha. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：FLISA IHC IP WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic linear peptide corresponding to an internal region of class I catalytic domain of PI3 Kinase that is specific to the p110 alpha isoform, conjugated to KLH.
