MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
he remodelling of the primitive vasculature in mammals to form the mature cardiovascular system includes a number of factors directing endothelial cell development.
Angiopoietin-1 is an angiogenic secreted protein that interacts with the endothelial specific Tie-2 receptor.
It is primarily expressed in developing endothelial cells.
During embryonic development, Ang-1 binds and induces tyrosine phosphorylation of Tie-2.
Ang-1 appears to play a crucial role in mediating matrix and mesenchyme.
It mediates blood vessel maturation and stability.
Ang-1 may play a critical role in heart development.
Ang-1 deficient mice mimic the phenotype exhibited by animals deficient in Tie-2.
A homolog of Ang-1, termed Angiopoietin-2 may act as an antagonist for Ang-1 and Tie-2.
Ang-1 and Ang-2 have ~60% sequence homology.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Specificity：Recognizes mouse Angiopoietin-1. Species Crossreactivity: human and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide selected from mouse Angiopoietin-1 (KLH). Cellular Localization: Secreted. Species sequence homology: bovine, canine, porcine, equine (95%).
