MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Sprouty is a suppressor of EGF and FGF signaling that appears to have a universal inhibitory role on tyrosine kinase signaling.
Evidence suggests that it does this by binding adapter proteins and GAP, thus inactivating Ras.
Sprouty expression is induced by the same pathways that it down-regulates, suggesting it plays a role in an auto-feedback loop.
Sprouty was originally identified as an inhibitor of Drosophila epidermal growth factor (EGF) and fibroblast growth factor (FGF) receptor signaling during tracheal development.
Four isoforms of mammalian sprouty are known (Spry1-Spry4).
The role of the mammalian analogs has not been clearly elucidated although it is believed that human Sprouty-2 (hSpry2) may be an inhibitor of cellular migration and proliferation.
Spry2 and Spry4 show considerable sequence homology between human and mouse at the C-terminus of the protein.
Significant sequence divergence occurs at the N-terminus.
Spry2 appears to be abundant in brain, lung and heart tissue, with lesser amounts found in kidney and skeletal muscle.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-1ug/ml detects Sprouty2 in RIPA lysates from HeLa and 3T3/A31 cells
Optimal dilutions to be determined by the researcher.
Included Positive Antigen Control:F4209: 3T3/A31 lysate.
Add 2.5ul of 2-mercaptoethanol per 100ul of lysate and boil for 5 minutes to reduce the preparation.
Load 10ug of reduced lysate per lane for minigels.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Specificity：Recognizes Sprouty2, Mr ~35-40kD. A non-specific protein was also detected, Mr ~70kD. Species Crossreactivity: Human and mouse. Crossreactivity with rat predicted, based on sequence homology.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：KLH-conjugated, synthetic peptide corresponding to aa26-39 (C-GEPDPRDALTQQVH) of human Sprouty2, with an N-terminalcysteine added for conjugation purposes. The immunizing sequence is identical in rat and mouse.
