84-3384-99　［取扱停止］REST (Repressor Element RE-1 Silencing Transcription Factor, NRSF, Neuronal Restricted Silencing Factor) (MaxLight 750) 100ul　R1623-ML750

特徴

• MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
• Absorbance (759nm); Emission (780nm); Extinction Coefficient 240,000.
• NRSF (neuron-restrictive silencer factor, also designated XBR and REST for RE1-silencing factor) is a silencer protein that binds the DNA sequence element NRSE (neuron-restrictive silencer element).
• The binding of NRSF to the NRSE represses neuronal gene transcription in non-neuronal cells.
• Although NRSF is most highly expressed in non-neural tissues, it is also expressed in developing neurons and at low levels in the brain.
• NRSF contains nine zinc finger domains, but also exists as a C-terminally truncated form produced by alternative splicing.
• This variant, REST4, contains five of the zinc-finger domains and weakly binds DNA, yet is transported to the nucleus.
• NRSF associates with mSin3 and HDAC in ventricular myocytes, suggesting a role for NRSF outside the nervous system.
• Down-regulation of NRSF, which normally occurs upon neural differentiation, is necessary for the proper development of certain classes of neurons.
• NRSF is required to repress neuronal gene expression in vivo, in both extra-neural and undifferentiated neural tissue.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:500- 1:2000.
• ES cell total extract was resolved by electrophoresis, transferred to nitrocellulose and probed with R1623 (1:1000).
• Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system
• Optimal dilutions to be determined by the researcher.
• Recommended Secondary Antibodies:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRbI1904-46Q: IgG, H&L (HRP) Pab Gt xRb
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: MaxLight™750 conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.