MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Applications:Suitable for use in Western Blot, Immunoprecipitation and IP-kinase assay.
Other applications not tested.
Recommended Dilution:Western Blot: detected cdk2 in nuclear extracts from HeLa cells
Immunoprecipitation: immunoprecipitated cdk2 from 500ug of HeLa nuclear extract
Immunoprecipitation Kinase Assay: 4ug of this lot immunoprecipitated active cdk2 from HeLa nuclear extract as shown by the ability to phosphorylate Histone H1
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Specificity：Recognizes human p33cdk2 protein kinase, Mr 33kD. Species Crossreactivity: Human. Predicted to crossreact with mouse, rat, non-human primates and hamster.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：KLH-conjugated, synthetic peptide corresponding to aa287-298 (CQDVTKPVPHLRL) of human cdk2, with a cysteine added for conjugation purposes.
