MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Calmodulin (CaM), a small 17 kDa ubiquitous Ca2+-binding protein acts as an intracellular Ca2+ receptor and transduces Ca2+ transients, usually in response to growth factors.
It mediates the activity of a number of Ca2+ regulating enzymes, including protein kinases, phosphatases, nitric oxide synthases, and phosphodiesterase.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:5000.
Detects a recombinant protein containing the calmodulin binding protein epitope tag in lysates from transformed E.
coli.
Lysates from E.
coli transformed with a plasmid encoding a GST-CBP, calmodulin binding protein epitope tagged.
Proteins were resolved by electrophoresis, transferred to nitrocellulose and probed with C1035-09.
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Specificity：Recognizes recombinant proteins containing the calmodulin binding protein epitope tag. Species sequence homology: rabbit, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Immunogen：A synthetic peptide, MKRRWKKNFIAVSAANRFKKISSSGAL corresponding to the Calmodulin Binding Protein (CBP) epitope tag found in several E. coli expression vectors (KLH).
