MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system.
It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilatation by releasing NO from the endothelium.
Unlike typical neurotransmitter, NO is a not stored in synaptic vesicle and does not act on membrane receptors.
Synthesis of NO, initially demonstrated in vascular endothelium, is now found in many tissues.
It is found in endothelium, adrenal glands, brain and platelets.
The other principle group is inducible, Ca2+/Calmodulin-independent, and inhibited by NMMA and L-NNA.
It has been found in macrophage, hepatocytes, tumor cells, vascular smooth muscle and endothelial cells.
Analyses of cDNA clones have identified three distinct NOS genes in mammals: neuronal (nNOS/bNOS/NOS-I), endothelial (eNOS/NOS-III), and macrophage (mNOS/iNOS/NOS-II).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500 detects affinity purified bacterial expressed rat nNOS/NOS I that had been phosphorylated by purified AMPK in vitro.
Lambda phosphatase treated and untreated affinity purified bacterial expressed rat nNOS/NOS I that had been phosphorylated by purified AMPK was resolved by electrophoresis, transferred to PVDF and probed with N5350-02 (1:500).
Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Specificity：Recognizes human phosphorylated nNOS/NOS I, Mr 170kD. Species Crossreactivity: rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to aa surrounding Ser1417 of human neuronal Nitric Oxide Synthase, with an N-terminal cysteine added for KLH conjugation purposes, where pS is phosphorylated serine corresponding to the AMPK phosphorylation site. Species Sequence Homology: mouse, zebrafish, frog. Sequence homology: Ser1451 of the nNOSu isoform.
