84-3383-99　［取扱停止］HEY 2 (Helix-loop-helix Transcription Factor, Hairy/enhancer-of-split related with YRPW motif 2, bHLHb32, BHLHB32, Cardiovascular helix-loop-helix factor 1, CHF1, Class B basic helix-loop-helix protein 32, GRIDLOCK, GRL, Hairy and enhancer of split-related protein 2, Hairy-related transcription factor 2, hCHF1, HERP, HERP1, HESR2, HESR-2, HES-related repressor protein 2, hHRT2, HRT2, HRT-2, MG ...　H2100-10-ML750

特徴

• MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
• Absorbance (759nm); Emission (780nm); Extinction Coefficient 240,000.
• The Hey family of bHLH transcription factors are direct targets of Notch signaling.
• HEY2 (gridlock) is an important determinant of mammalian heart development, loss of which in mouse leads to cardiac defects with high postnatal lethality.
• Mouse Hey1 knockouts have no apparent phenotypic defect but the combined loss of Hey1 and Hey2 results in embryonic death after embryonic day 9.5 (E9.5) with a global lack of vascular remodeling and massive hemorrhage.
• Similar defects are observed in Jagged1 and Notch1 knockout mice.
• Applications:Suitable for use in Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Immunohistochemistry Protocol:1.
• Dissect tissues.
• Freeze on dry ice
• 2.
• Cut on a cryostat - 10um slices
• 3.
• Dry slides at RT for 30-90 min
• 4.
• Fix with cold Acetone:Methanol (50:50) for 2 min.
• Either process further or store at -20°C.
• Alternative is fixation with 4% PFA and treatment with a 0.05% trypsin solution.
• Incubate for 10 min.
• at 37°C.
• Followed by 3 washes with PBS
• 5.
• Let air dry
• 6.
• Treat with PBS for 5 min
• 7.
• Incubate in 50mM ammonium chloride for 30 min
• 8.
• Treat with PBS for 5 min
• 9.
• Treat with Blocking serum for 30-45 min
• 10.
• Primary antibody, H2100-10, for 90 min.
• Dilution: 1:100-1:60011.
• Wash 3 times for 5 min
• 12.
• Secondary fluorescent conjugated antibody for 30 min
• 13.
• Wash 3 times for 5 min.
• and mount.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: MaxLight™750 conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.