MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Western blot: 1:10 using ECL Immunoprecipitation: 1:10 following the protocol of Raymond et al, Nature (1993) 361:637–641.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Affinity Purified
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Specificity：Recognizes glycine receptor alpha 1. Shows crossreactivity to glycine receptor alpha 2. subunits Used successfully in Western blot and immunoprecipitation assays with cell extracts containing recombinant human glycine receptors and with homogenates of mouse spinal cord. By Western blot recognizes a protein with a MW of ~48kD. Species Reactivity: Human and mouse
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mo
Immunogen：Peptide from the amino terminus of the human alpha 1 glycine receptor subunit conjugated to KLH.
