MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Recent studies indicate that the expression of CD44 on the cell surface changes profoundly during tumor metastasis, particularly during the progression of various carcinomas.
Sequencing analysis reveals that CD44 exists in different forms (at least 15 isoforms have been identified) that are variants of the common or standard hemopoietic form CD44s.
Specifically, the human CD44 gene contains 19 exons, out of which 10 exons can potentially be alternatively spliced.
In particular, CD44s contains exons 1–15 and exons 15–19.
The other exons (e.g.
6–14) are also spliced in a variety of different cells.
For example, epithelial cells contain exons 12–14 inserted into the CD44s transcripts.
This isoform has been designated as CD44E.
Other CD44 variants CD44v) have the same sequences at the two ends of the molecule, but differ in a middle region (with different numbers of additional exons) located on the external side of the cell membrane.
It has been suggested that expression of CD44 variants with the additional exon 11 (so-called v7) is responsible for tumor progression and metastasis.
In fact, CD44v7 expression has been used as an indicator of metastasis.
Applications:Suitable for use in FLISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot and RIA.
Another potential application, though not tested, is Direct Flow Cytometry.
Other applications have not been tested.
Recommended Dilution:Optimal dilution determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by ammonium sulfate precipitation.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Specificity：Recognizes human CD44v7 (~200-250kD). Species Crossreactivity: rabbit, porcine, mouse, rat, canine and bovine.
Isotype：IgG
Calc Applications Abbrev：FLISA IF IHC IP RIA WB
Calc Crossreactivity：Bo Ca Hu Mo Po Rb Rt
Immunogen：Synthetic peptide corresponding to human CD44v7.
