MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Two types of PDGF receptors have been identified: PDGF Receptor alpha (PDGFRa) and PDGF Receptor beta(PDGFRb).
The PDGF Receptor alpha, a 170kD protein, binds all three isoforms of PDGF with high affinity whereas the PDGF Receptor beta, a 190kD protein, appears to bind only the PDGF BB homodimer with high affinity.
Both receptors are transmembranous, ligand activated protein tyrosine kinases, which phosphorylate a number of important signal transduction proteins, which are bound with differential affinities via SH2 domains.
The response of any given cell to PDGF will depend on the types of receptors displayed on the surface and isoforms of PDGF present in the extracellular environment
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C.
For long-term storage, aliquot and store at 4°C.
Do not freeze.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Specificity：Recognizes human Platelet Derived Growth Factor Receptor alpha, doublet, Mr 150/170kD. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa1035-1053, C-GKRNRHSSQTSEESAIETG of human Platelet Derived Growth Factor Receptor alpha, conjugated to KLH. Species Sequence Homology: frog, chicken, rat and zebra fish, which is different from the human sequence by one amino acid.
