MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
PKCe is a member of the novel protein kinase C family of serine/threonine kinases.
Its activity is calcium-independent and phospholipid-dependent and is expressed at low levels in normal tissue except the brain, where expression is higher.
PKCe expression appears to be induced by interferon-a and its over-expression may play a role in tumorigenesis.
PKCe, through its regulatory domain, can induce immature neurite-like processes via a mechanism that appears to be of importance for neurite outgrowth during neuronal differentiation.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/ml detects PKCe in Jurkat cell lysate.
Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with P9103-20C PKCe (0.5ug/ml).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by researcher.
Included Positive Antigen Control: (J9000)Jurkat cell lysate.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Specificity：Recognizes mouse PKCe, Mr 95kD. Species Crossreactivity: human and rat.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide, aa726-737 (KGFSYFGEDLMP), corresponding to the C-terminal variable (V5) region of mouse PKCe (KLH). Species sequence homology: rabbit.
