MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection.
STAT (signal transducers and activators of transcription) proteins are transcription factors that are selectively phosphorylated on tyrosine residues by Jak family kinases and some receptors with tyrosine kinase activity.
The STAT proteins have highly conserved amino termini.
STAT family members diverge in their carboxy termini.
STAT6 is activated by cytokines, IL-4 and IL-3.
Evidence shows that STAT6 becomes activated afterPDGF stimulation of NIH 3T3 fibroblasts.
STAT6 is distinguishable from the other STAT family members by its ability to bind a GAS-like element found in the Ig germ line e promoter of the IL-4 responsive human Ce gene (Ie).
Applications:Western Blotting: 1:1000 dilution detects phospho-STAT6 in lysates of Daudi cellsstimulated with rhIL-4.
100ug of lysate from quiescent (lane 1) or rhIL-4 stimulated Daudi cells (lane 2) was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-STAT6 (1:1000).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™650 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Specificity：Recognizes phospho-STAT6, Mr ~100kD; no Crossreactivity with unphosphorylatedSTAT6; recognizes a non-specific band at 30kD. Species Crossreactivity: Human and mouse, other species not determined.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to amino acids 631-645 of human STAT6 (CPEQMGKDGRGpYVPAT).
