84-3380-89　［取扱停止］STAT 2 (Signal Transducer and Activator of Transcription 2) (MaxLight 650) 100ul　S7970-86-ML650

特徴

• MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection.
• Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
• Applications:Suitable for use in Western Blotting, Immunoprecipitation, EMSA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5-2ug/ul.
• 3T3 cell lysate probed with anti-STAT2 (0.5ug/ul)
• Immunocytochemistry: Positive immunostaining reported on fresh cells fixed with 3.7% formaldehyde and permeabilized with 0.2% Triton1
• EMSA: Supershift STAT2 containing complexesOptimal dilutions to be determined by the researcher.
• Positive Antigen Control: 3T3 lysate.
• Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation.
• Load 20ug of reduced lysate per lane for minigels.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: MaxLight™650 conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.