84-3380-73　［取扱停止］CPI-17 (PKC Potentiated Inhibitor Protein-17) (MaxLight 650) 100ul　C7905-50-ML650

特徴

• MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection.
• Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
• PKC Potentiated Inhibitor Protein-17 (CPI-17) is a 17kD protein.
• Expressed in smooth muscles and neuronal cells, CPI-17 is a myosin phosphatase inhibitor protein.
• Phosphorylation of CPI-17 at Thr38 enhances its inhibitory effect by 100 fold.
• Once phosphorylated, CPI-17 inhibits PP1c and MLCP holoenzyme activity.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: Detects CPI-17 in L6 lysates.
• L6 lystate was resolved by electrophoresis, transferred to nitrocellulose and probed with C7905-50.
• Proteins were visualized using an IgG (HRP) goat anti-rabbit secondary antibody and a chemiluminescence detection system
• Optimal dilutions to be determined by the researcher.
• Recommended Secondary Antibodies:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x RbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x RbI1904-46Q: IgG, H&L (HRP) Pab Gt x Rb
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: MaxLight™650 conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.