MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection.
Calcineurin/PP2B Aβ, also known as Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform, and encoded by the gene PPP3CB/CALNA2/CALNB/CNA2, is a calcium dependent, calmodulin stimulated catalytic subunit protein phosphatase critical in the function of Calcineurin an important phosphatase governing immune responses such as T cell activation and the T cell immune response.
Calcineurin is a heterodimeric protein consisting of a catalytic subunit Calcineurin A, which contains an active site dinuclear metal center, and a tightly associated, myristoylated, Ca(2+)-binding subunit, Calcineurin B.
Calcineurin/PP2B Aβ is the beta isoform of the catalytic active site of the subunit A of Calcineurin.
Calcineurin also plays pivotal roles in signaling transduction cascades in a variety of other immune cells as well as non-immune cells like myocytes and neurons.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: detects Calcineurin A/PP2B catalytic unit in 10ug of human brain tissue lysate
Immunoprecipitation: immunoprecipitated Calcineurin A/PP2B catalytic unit in 500ug of mouse brain tissue lysate
Optimal dilution to be determined by researcher
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™650 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Specificity：Recognizes rat Calcineurin/PP2B A beta. Species Crossreactivity: human and mouse
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：KLH-conjugated linear peptide corresponding to rat Calcineurin A/PP2B catalytic unit near the C-terminus.
