MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection.
S-Adenosyl-I-methionine-dependent protein arginine N-methyltransferases (PRMTs) methylate arginine residues within a variety of proteins, particularly histones.
At least four distinct mammalian family members have now been described, including PRMT1, PRMT3, CARM1/PRMT4 and JBP1/PRMT5
Applications:Suitable for use in Western Blotting, FLISA.
Other applications not tested.
Recommended Dilution:Western Blot: Reacts with a band of approximately 57kD on whole HL60 cell lysate.
Additional bands may be present depending on sample used
FLISA (direct): 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™650 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified immunoglobulin
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Specificity：Recognizes human PRMT3 (Protein Arginine N-Methytransferase 3).
Isotype：IgG
Calc Applications Abbrev：FLISA WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide from N-terminus of human PRMT3.
