MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection.
All cells within tissues are surrounded by an extracellular matrix (ECM) giving the tissues shape and structure.
The ECM is constantly being remodeled and constant communication is maintained between cells through this matrix.
Secreted proteins, termed matrix metalloproteinases (MMPs), are involved in the modulation of cell matrix interactions.
MMPs are Zn(2+) binding endopeptidases that degrade various components of the ECM.
MMPs are enzymes implicated in normal and pathologic tissue remodeling processes, wound healing, angiogenesis, and tumor invasion.
MMP9, also known as gelatinase B, is a secreted enzyme which degrades the interstitial collagens, types I, II, and III, and is produced by normal alveolar macrophages and granulocytes.
The expression of MMP9 increases in Epstein-Barr virus infected lymphoma derived cell lines and may be of significance in typically invasive nasopharyngeal carcinomas.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilutions:mmunohistochemistry: Frozen acetone fixed tissuesOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™650 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Specificity：Recognizes rat Matrix Metalloproteinase 9 pro and active forms. Exhibits no crossreactivity with other MMP family members. Species Crossreactivity: mouse and human
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：E. coli expressed active rat 92kD type IV collagenase (catalytic domain).
