MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 0.5-1ug/ml detected histone H3on 10ug of Colemid treated HeLa acid extract.
Colemid treated HeLa acid was resolved by electrophoresis, transferred to PVDF and probed with H5110-14B.
Proteins were visualized using a goat anti-rabbit secondary antibody (HRP) and a chemiluminescence detection system
Immunoprecipitation: 10ug specifically immunoprecipitated phosphorylated histone H3 from HeLa acid extract and Colcemid treated HeLa acid extracted RIPA lysate
Immunocytochemistry: 1:500 detected Histone H3 in HeLa and A431 cells
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™650 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Specificity：Recognizes human histone H3 phosphorylated at Serine 10 at ~17kD. Species Crossreactivity: mouse. Broad species reactivity expected due to sequence homology.
Isotype：IgG
Calc Applications Abbrev：IC IP WB
Calc Crossreactivity：Dr Hu Th Xe
Immunogen：Linear peptide coresponding to human Histone H3 at Ser10
