84-3379-71　［取扱停止］Superoxide Dismutase, Mn (SOD) (MaxLight 650) 100ul　S8060-10A-ML650

特徴

• MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection.
• Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
• Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS).
• SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase.
• Several classes of SOD have been identified.
• These include intracellular copper, zinc SOD (Cu, Zn SOD/SOD1), mitochondrial manganese SOD (Mn SOD/SOD2) and extracellular Cu, Zn SOD (EC SOD/SOD3).
• The manganese containing 80kD tetrameric enzyme SOD2 is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications have not been tested.
• Recommended Dilutions:Western Blot: Detects MnSOD in RIPA lysates of rat L6 RIPA cells.
• L6 cell lysates were resolved by electrophoresis and transferred to nitrocellulose probed with S8060-10A.
• Proteins were visualized using an IgG (HRP) Pab Gt xRb and a chemiluminescence detection system
• Immunohistochemistry(paraffin): Detected MnSOD in paraffin embedded rat brain tissue sections
• Optimal dilutions to be determined by the researcher.
• Recommended Secondary Antibodies:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRbI1904-46Q: IgG, H&L (HRP) Pab Gt xRb
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: MaxLight™650 conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.