MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection.
Acetylation and deacetylation of core histone lysine residues, by histone acetyltransferases (HAT) and histone deacetylases (HDAC) respectively, have been shown to be necessary for some crucial nuclear events such as gene transcription, DNA replication and repair.
HDACs are found in multi-protein complexes with transcriptional co-repressors such as mSin3, RbAp46/48, SMRT, and NCoR.
HDAC3, which is over 50% identical to both HDAC1 and HDAC2, is ubiquitously expressed and exhibits histone deacetylase activity, represses transcription and associates with the transcription factor YY1.
The identification of a family of functionally redundant HDACs suggests each may have a unique function, supported by the sequence divergence of the carboxy-termini.
Applications: Immunoblot Analysis: 1–4ug/ml detected HDAC3 from a HeLa RIPA cell lysate (1 hour exposure).
4ug/ml detected HDAC from mouse WEHI and rat L6 RIPA cell lysates (30 minute exposure).
Immunoblot Analysis HeLa cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-HDAC3 (1ug/ml).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™650 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Specificity：Recognizes human HDAC3 at 49kD. Species crossreactivity: mouse and rat. Expected to crossreact with chicken based on sequence identity.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Ch Hu Mo Rt
Immunogen：KLH-conjugated, 18 residue synthetic peptide corresponding to amino acids 411–428 of human HDAC3 (NEFYDGDHDNDKESDVEI). The immunizing sequence is identical for chicken and for the first 14 residues in mouse.
