MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection.
Spinophilin/Neurabin-II has been found to be enriched at the postsynaptic density fraction in the brain and in the adherens junction fraction in rat liver.
Within the brain, Spinophilin/Neurabin-II is localized in the heads of dendritic spines.
Spinophilin/Neurabin-II contains a single PDZ domain in the middle region of the protein and other domains that predict it will form a coiled-coil structure at the C-terminus.
Spinophilin/Neurabin-II is known to complex with the catalytic subunit of protein phosphatase I (PP1) and in vitro is a potent modulator of PP1 enzymatic activity.
Spinophilin/Neurabin-II is also known to interact with F-actin and rat lin-10.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™650 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Specificity：Recognizes rat spinophilin at ~120kD. Also recognizes a band at ~95kD which is suspected to be a cleavage product of spinophilin.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Rt
Immunogen：GST fusion protein corresponding to residues 286-390 of rat brain Spinophilin
