Bak or Bcl2 homologous antagonist is a member of the Bcl2 family of proteins.
The Bcl-2 related proteins interact with one another through the formation of homo and heterodimers.
The susceptibility of cells to apoptotic stimuli is thought to be controlled by the relative ratios of the different Bcl2 family proteins.
Bak has been demonstrated to accelerate the rate of apoptosis in growth factor deprived murine lymphoid, neuronal and fibroblastic cell lines.
This protein localizes to mitochondria, and functions to induce apoptosis.
It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c.
Bak also interacts with the tumor suppressor P53 after exposure to cell stress.
Applications:Suitable for use in Western Blot, Immunohistochemistry, immunocytochemistry and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilution:Immunohistochemistry: Paraffin sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™650 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Specificity：Recognizes human Bak. Species Crossreactivity: rat and mouse
Isotype：IgG
Calc Applications Abbrev：IC IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acid residues 23-38 (SEEQVAQQDTEEVFRSC) of human Bak with a cysteine residue added to the C-terminus for conjugation to KLH.
