MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection.
p52 is a subunit of the nuclear factor NF Kappa-B, which binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions.
The transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses.
In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory protein known as IkB.
An impressive variety of stimuli (tumor necrosis factor, interleukin 1, T-cell activation signals, bacterial endotoxins, viral transforming proteins, certain growth factors and reactive oxygen intermediates) lead to the rapid nuclear accumulation of NFkB by the induced phosphorylation and subsequent degradation of IkB.
In the nucleus, NFkB regulates genes encoding cytokines, cytokine receptors, cell adhesion molecules, proteins involved in coagulation and genes involved in cell growth control.
Additionally, NFkB is thought to be an important transcriptional regulator for HIV.
Growing evidence indicates that the dysregulation of NFkB may be key to a number of diseases including arthritis and other inflammatory diseases, Alzheimer's disease, atherosclerosis and cancer.
NFkB is formed through the association of multiple subunits, either as a homodimer or heterodimer.
Subunits have been identified as p50 (NFkB1), p65 (RelA), c-Rel, RelB and p52 (NFkB2).
The classic NFkB form exists as a p50-p65 heterodimer and predominates in many cell types.
Many of the possible combinatorial forms of homo- and heterodimers have been identified and growing evidence indicates that different forms of NFkB have different functions in cells.
Interestingly, both the p50 and p52 subunits are derived from the precursor proteins p105 and p100 respectively, that each contain multiple copies of the so-called ankyrin repeat at their C-termini.
Nuclear translocation of NFkB is confirmed by the use of electrophorectic mobility shift assays or by immunoblotting with nuclear extracts.
The subunit composition of NFkB is confirmed by the use of antibodies that "supershift" the DNA/protein complex.
Applications:Suitable for use in Immunoprecipitation and Western Blot.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:1000 dilution detected NF KB, p52 and its 110kDa precursor in RIPA lysates from Raji cells.
Raji cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-NF KB, p52 (1:1000).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation: 4ul immunoprecipitated NF KB, p52 and its 110kDa precursor from 500ug of a Raji RIPA cell lysate.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Specificity：Recognizes human NF KB, p52, (52kD) and its 110kD precursor.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to the N-terminal region of human NF-kB, p52 (KLH).
