MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection.
Raf-1 associates with members of the 14–3–3 family of proteins, which appear to protect active Raf-1 from phosphatase action.
Important regulatory phosphorylation events occur on tyrosines 340 and 341 and serines 259, 499 (activating), serine 43 (prevents Ras:GTP binding), and serine 621 (constitutive, required for activity).
Once active, Raf-1 associates with plasma membranes independent of Ras and phosphorylates and activates MEK, initiating the MAP kinase signaling pathway.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™650 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Specificity：Recognizes human Raf-1, Mr ~70kD. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa637-648, CTLTTSPRLPVF of human Raf-1, conjugated to KLH. Species Sequence Homology: rat
