MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells.
Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO.
NO production is mediated by members of the nitric oxide synthase (NOS) family.
NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO.
Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned.
Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels.
Protein kinase C-stimulating agents exhibit the same effect on iNOS activity.
Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5ug/mlImmunoprecipitation: 10ug per 500ug treated cell lysateImmunohistochemistry(Paraffin): 1:50 - 1:2:00 Optimal dilutions to be determined by the researcher.
Positive Control: IFNgamma/LPS untreated and treated RAW264.7 cell lysates
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity：Recognizes mouse NOS II, Mr ~125kD. Species Crossreactivity: human
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to the N-terminus of mouse iNOS/NOS II, GST-tagged. Species Sequence Homology: Rat (85%)
