MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
Type 1 protein Ser/Thr phosphatases (PP1) have a high specific activity against phosphorylase, and are resistant to okadaic acid up to concentrations of 50 nM, but sensitive to Inhibitor-2 and microcystin LR.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Positive Control:HeLa Cell Lysate
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity：Recognizes human Protein Phosphatase 1 gamma1. No cross reactivity with other recombinant PP1 isoforms. Species Crossreactivity: Mouse, Rat, and Bovine.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Bo Hu Mo Rt
Immunogen：Synthetic peptide corresponding to C –terminus of PP1 gamma 1 catalytic subunit (aa311-323).
